Biochemical Assays

The following list of FRET assays can be employed for general or selectivity screening purposes.

List of available biochemical assays (PDF)

For detailed information or a quotation please send a mail to info@phenex-pharma.com

 

FRET Assay System

Phenex uses Nuclear Receptor Ligand Binding Domains, fused to GST as one binding partner and biotinylated peptides with sequences around the Cofactors LXXLL motifs as the other binding partner.

The FRET assay principle is the energy transfer, when Eu-chelate as fluorescent donor and Allophycocyanin as fluorescent acceptor come close to each other.
If the titrated ligand modulates the interaction between Nuclear Receptor and peptide, the fluorescent signal is modulated in a ligand dose dependent manner.

Figure - Principle of FRET Assay System

Application examples for the FRET system:

- Liver X Receptor alpha (LXRa) plus SRC1 peptide with control agonist T0901317
- Liver X Receptor beta (LXRb) plus SRC1 and NCoR peptide with control agonist T0901317
- Farnesoid X Receptor (FXR) plus SRC1 peptide with control agonist GW4064
- Peroxisomeproliferator Activated Receptor gamma (PPARg) plus SRC1 peptide in Agonist Mode with control agonist Rosiglitazone and in Antagonist Mode with control antagonist GW9662