Y2H - Compound selective cofactor recruitment by the ERα

The compound concentrations increasing from left to right.
Main results:

• coumestrol differs from estradiol mainly in potency.
• hexestrol clearly promotes CF9 recruitment.
• genistein is of lower potency than estradiol and does not promote ERα-dimerisation.
• ICI172,780 does not induce CF-recruitment, but clearly supports ERα-dimerisation.

 

17β-estradiol

coumestrol

hexestrol

genistein

ICI172,780